Quorum-sensing signal production byAgrobacteriumvitis strains and their tumor-inducing and tartrate-catabolic plasmids

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Title: Quorum-sensing signal production byAgrobacteriumvitis strains and their tumor-inducing and tartrate-catabolic plasmids
Author: Lowe, Nathanial; Gan, Han Ming; Chakravartty, Vandana; Scott, Russell; Szegedi, Erno
Abstract: Agrobacterium vitis strains, their tumor-inducing (pTi) and tartrate utilization (pTr) plasmid transconjugants and grapevine tumors were analyzed for the presence of N-acyl-homoserine lactones (AHLs). All wild-type A. vitis strains produced long-chain signals. PCR analysis of the A. vitis long-chain AHL synthase gene, avsI, showed the predicted amplicon. Agrobacterium tumefaciens UBAPF2 harboring various A. vitis pTi plasmids produced N-(3-oxo-octanoyl)-l-homoserine lactone encoded also by pTis of A. tumefaciens. UBAPF2 transconjugants carrying pTrs except for pTrTm4 and pTrAB3, also produced an AHL. UBAPF2 transconjugants carrying pTrAT6, pTrAB4 and pTrRr4 or pTiNi1 produced two additional AHLs not observed in the corresponding wild-type strains. We also provide evidence for in situ production of AHLs in grapevine crown gall tumors of greenhouse and field origin.
Description: 2009 article from the July, 2009 issue of FEMS Microbiology Letters. The definitive version is available at www.blackwell-synergy.com
Record URI: http://hdl.handle.net/1850/10234
Date: 2009-07

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