Identification of an rsh gene from a novosphingobium sp. necessary for quorum-sensing signal accumulation

Show simple item record Gan, Han Ming Buckley, Larry Szegedi, Erno Hudson, André O. Savka, Michael A. 2009-07-22T16:12:51Z 2009-07-22T16:12:51Z 2009-04
dc.description April 2009 article from the journal of bacteriology. Please see for more information. en_US
dc.description.abstract The stringent response is a mechanism by which bacteria adapt to environmental stresses and nutritional deficiencies through the synthesis and hydrolysis of (p)ppGpp by RelA/SpoT enzymes. Alphaproteobacteria and plants contain a single Rsh enzyme (named for RelA/SpoT homolog) that is bifunctional. Here we report the identification of a new species of bacteria belonging to the genus Novosphingobium and characterization of an rsh mutation in this plant tumor-associated isolate. Isolate Rr 2-17, from a grapevine crown gall tumor, is a member of the Novosphingobium genus that produces the N-acyl-homoserine lactone (AHL) quorum-sensing (QS) signals. A Tn5 mutant, Hx 699, deficient in AHL production was found to have an insertion in an rsh gene. The Rsh protein showed significant percent sequence identity to Rsh proteins of alphaproteobacteria. The Novosphingobium sp. rsh gene (rshNsp) complemented the multiple amino acid requirements of the Escherichia coli relA spoT double mutant by restoring the growth on selection media. Besides QS signal production, the rsh mutation also affects soluble polysaccharide production and cell aggregation. Genetic complementation of the Hx 699 mutant with the rshNsp gene restored these phenotypes. This is the first discovery of a functional rsh gene in a member of the Novosphingobium genus. en_US
dc.language.iso en_US en_US
dc.publisher ASM - Journal of Bacteriology en_US
dc.relation RIT Scholars content from RIT Digital Media Library has moved from to RIT Scholar Works, please update your feeds & links!
dc.relation.ispartofseries Vol. 191 en_US
dc.relation.ispartofseries No. 8 en_US
dc.subject Novosphingobium en_US
dc.subject Sphingomonas en_US
dc.title Identification of an rsh gene from a novosphingobium sp. necessary for quorum-sensing signal accumulation en_US
dc.type Article en_US

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