Determination of the neighboring molecule to the FC receptor on human macrophages

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Title: Determination of the neighboring molecule to the FC receptor on human macrophages
Author: Malinski, Lorri Jean
Abstract: Chemical cross-linking studies have been carried out to investigate near neighbors to the receptor for immunoglobulin G[lgG] on U937 cells both before and after solubilization of the receptor. A cross-linked product with a molecular weight of 350.000 daltons was achieved with. DMS on intact and lysed U937 cells, DTBP on intact cells and DSS on cell lysates, with anti-FcR as the immunoprecipitation reagent. Following cross-linking of the U937 cells with DTBP and analysis by two-dimensional gel electrophoresis the p350 molecule appeared to be a p 1 70 dimer. The pl70 molecular has been related to some nonspecificity of the anti-FcR. These procedures were thus unsuccessful in identifying a neighboring molecule to the U937 cell FcR. DSS cross-linking of Fc fragments of IgG and Fab fragments of anti-FcR to the FcR on intact U937 cells was unsuccessful. Lack of success in this regard is more likely attributed to difficulties in DSS usage then to inability to cross-link close-lying molecules to the FcR. The investigations presented clarify the problems that must be overcome before successful cross-linking can be achieved between the U937 cell FcR and its neighboring molecule [s].
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Date: 1984

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