Preparation of a bank of cloned genes from the chromosome of Agrobacterium tumefaciens and the isolation of genes involved in DNA repair and genetic recombination

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dc.contributor.advisor Rothman, Robert
dc.contributor.advisor Harrison, Howard
dc.contributor.advisor Aumer, James
dc.contributor.author Bartholomeusz, Geoffrey A.
dc.date.accessioned 2010-02-03T22:17:58Z
dc.date.available 2010-02-03T22:17:58Z
dc.date.issued 1990-06-25
dc.identifier.uri http://hdl.handle.net/1850/11436
dc.description.abstract The virulent property of the Agrobacterium tumefaciens is associated with its tumor inducing (Ti) plasmid. Recent studies on the virulence of this bacterium has shown that genes located on its chromosome also contribute to this property. One such gene is thought to produce a protein that has properties similar to that of the recA protein of E. colt. This thesis outlines the techniques that were used to try and isolate the recA-like gene from the chromosome of the Agrobacterium tumefaciens. All the techniques used in this study are outlined in detail and an explanation given for the choice of each technique. For reasons not completely understood, we were unable to isolate the recA-like gene even though a gene bank was successfully constructed and appropriately sized fragments extracted. Attempts were made to explain some of the unexpected results and appropriate steps were proposed to further understand them. en_US
dc.language.iso en_US en_US
dc.subject Cytogenetics en_US
dc.subject.lcc QH434 .B377 1990
dc.subject.lcsh Cytogenetics en_US
dc.subject.lcsh Gram-negative bacteria en_US
dc.subject.lcsh DNA repair en_US
dc.title Preparation of a bank of cloned genes from the chromosome of Agrobacterium tumefaciens and the isolation of genes involved in DNA repair and genetic recombination en_US
dc.type Thesis en_US
dc.description.college College of Science en_US
dc.description.department Department of Medical Sciences en_US

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