Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western New York state

Show simple item record

dc.contributor.advisor Takacs, Gerald - Chair en_US
dc.contributor.author Malatova, Katarina en_US
dc.date.accessioned 2005-12-16T19:35:43Z en_US
dc.date.accessioned 2006-03-13T14:00:12Z en_US
dc.date.available 2005-12-16T19:35:43Z en_US
dc.date.available 2006-03-13T14:00:12Z en_US
dc.date.issued 2005-12-16T19:35:43Z en_US
dc.identifier.uri http://hdl.handle.net/1850/1186 en_US
dc.description.abstract Screening of hydrocarbon degrading microorganisms isolated from three habitats in Western New York State by selective enrichment technique, resulted in the collection of 20 distinct species. All strains were cultivated in liquid media with crude oil as a sole carbon and energy source. Bacterial strains capable of degrading hydrocarbons belong to the genera Serratia marcescens, Acinetobacter baumannii and Pseudomonas sp. Carbon dioxide evolution experiments were used as the major indicator of microbial degradation of oil in biometric flasks. The measurements of CO2 evolution rates have shown to effectively evaluate the biodegradation rates by providing significant data within a short period. The chemical composition of the residual oil was determined by gas-chromatographic techniques. The results indicate that the highest accumulation of carbon dioxide and the highest degradation efficiency of medium chain alkanes were observed on Leepershank crude oil, whereas degradation of Mexican crude oil with a composition of higher saturated and substituted hydrocarbons was prolonged. Additionally, biodegradation of Smakover oil was significantly reduced due to a high content of aromatic and cyclic hydrocarbons. Noticeable formation of solubilizing agents was observed by GR1 (not yet identified clone) and Serratia marcescens. The results also suggest that the application of bacterial consortiums containing combinations of two isolated strains enhanced the degradation of Mexican crude oil and also lead to a successful utilization of complex organic industrial waste. The bacterial mixture of GR1 clone and Acinetobacter baumannii demonstrated the highest growth and CO2 evolution on both substrates among all tested bacterial blends. en_US
dc.format.extent 388456 bytes en_US
dc.format.extent 1753404 bytes en_US
dc.format.extent 147635 bytes en_US
dc.format.extent 402 bytes en_US
dc.format.extent 2061 bytes en_US
dc.format.extent 676 bytes en_US
dc.format.extent 7743 bytes en_US
dc.format.extent 49 bytes en_US
dc.format.extent 388456 bytes en_US
dc.format.extent 1753404 bytes en_US
dc.format.mimetype application/pdf en_US
dc.format.mimetype application/pdf en_US
dc.format.mimetype text/plain en_US
dc.format.mimetype text/plain en_US
dc.format.mimetype text/plain en_US
dc.format.mimetype application/octet-stream en_US
dc.format.mimetype application/octet-stream en_US
dc.format.mimetype application/octet-stream en_US
dc.format.mimetype application/pdf en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.subject Biodegradation en_US
dc.subject CO2 evolution en_US
dc.subject Crude oil en_US
dc.subject Hydrocarbon-degrading bacteria en_US
dc.subject.lcc TD192.5 .M35 2005 en_US
dc.subject.lcsh Bioremediation--New York (State) en_US
dc.subject.lcsh Hydrocarbons--Biodegradation--New York (State) en_US
dc.subject.lcsh Petroleum--Biodegradation--New York (State) en_US
dc.subject.lcsh Bacteriology--Cultures and culture media en_US
dc.subject.lcsh Anaerobic bacteria en_US
dc.title Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western New York state en_US
dc.type Thesis en_US
dc.description.college College of Science en_US
dc.description.department Department of Chemistry en_US
dc.description.approval 2005-11 en_US

Files in this item

Files Size Format View
KMalatovaThesis112005.pdf 1.753Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search RIT DML


Advanced Search

Browse