Toward the development of an analysis methodology for the elucidation of proteome differentiation between two strains of Saccharomyces cerevisiae

Show simple item record

dc.contributor.advisor Kim, Thomas
dc.contributor.advisor Evans, Irene
dc.contributor.advisor Aumer, James
dc.contributor.author Carter, Stephanie M. Prosa
dc.date.accessioned 2010-12-22T17:56:22Z
dc.date.available 2010-12-22T17:56:22Z
dc.date.issued 2008-12-08
dc.identifier.uri http://hdl.handle.net/1850/13069
dc.description.abstract Through continued development, the two-dimensional PAGE gel technique developed by O'Farrell in 1975 has emerged as the hallmark technology utilized in virtually all protein expression analyses. The principle objective of this study was the development and validation of a proteome analysis methodology using this technique suitable for use in determining the quantifiable proteome differences between prion containing [PSI+] and wild type [psi-] strains of Saccharomyces cerevisiae. In addition to the development of an assay, which yields highly reproducible whole proteome isolates from both strains, was the elicitation of statistically significant proteome changes between the two strains. Thus, this study concludes that there is a quantifiable and reproducible effect on protein expression conveyed by the prion state in yeast, which warrants future studies to realize this implication within the context of epigenetics. Future studies will further benefit via the implementation of the methodology proven viable in this work in determining the specific nature of the observed proteome differential expression. en_US
dc.language.iso en_US en_US
dc.subject 2d-page en_US
dc.subject Electrophoresis en_US
dc.subject IEF en_US
dc.subject Prion en_US
dc.subject Proteome en_US
dc.subject Saccharomyces cerevisiae en_US
dc.subject.lcc QK623.S23 C37 2008
dc.subject.lcsh Saccharomyces cerevisiae--Genetics en_US
dc.subject.lcsh Proteomics en_US
dc.subject.lcsh Prions en_US
dc.title Toward the development of an analysis methodology for the elucidation of proteome differentiation between two strains of Saccharomyces cerevisiae en_US
dc.type Thesis en_US
dc.description.college College of Science en_US
dc.description.department Department of Medical Sciences en_US

Files in this item

Files Size Format View
SProsaCarterThesis12-8-2008.pdf 3.635Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search RIT DML


Advanced Search

Browse