Quantitation of yohimbine utilizing capillary electrophoresis. Method development through statistical design.

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Title: Quantitation of yohimbine utilizing capillary electrophoresis. Method development through statistical design.
Author: Cooper, Jonathan
Abstract: In an effort to combat the rising cost of health care in the United States by encouraging preventative and self-care, the federal government passed the Dietary Supplement Health Education Act of 1994. This act essentially deregulated the natural product market allowing herbs, including yohimbine, to be sold over the counter without undergoing the same strict evaluation as a traditional synthetic drug candidate. As the full potential of these natural products is realized, including possible harmful side effects, a need arises for the identification and quantitation of the active components from commercial preparations. Commercial samples were qualitatively and quantitatively analyzed for yohimbine utilizing a capillary electrophoretic method optimized through statistical design. Calibration curves produced with this method routinely achieved R2 > 0.998. Method reproducibility (n = 10) and extraction efficiency (99.8% recovery) were determined to be sufficient. It was shown that a statistical design could limit the amount of experimental runs while providing a significant amount of information about experimental parameters.
Record URI: http://hdl.handle.net/1850/13941
Date: 1999-11

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