Determination of free progesterone in an ultrafiltrate of saliva collected in situ

Show simple item record

dc.contributor.author Schramm, W. en_US
dc.contributor.author Smith, R. en_US
dc.contributor.author Craig, Paul en_US
dc.contributor.author Paek, S. en_US
dc.contributor.author Kuo, H. en_US
dc.date.accessioned 2006-07-19T19:35:02Z en_US
dc.date.available 2006-07-19T19:35:02Z en_US
dc.date.issued 1990-08-01 en_US
dc.identifier.citation Clinical Chemistry 36N8 (1990) 1488-1493 en_US
dc.identifier.issn 1530-8561 en_US
dc.identifier.uri http://hdl.handle.net/1850/2154 en_US
dc.description Article may be found at: http://www.clinchem.org/cgi/content/abstract/36/8/1488 en_US
dc.description.abstract We have investigated the utility of an ultrafiltrate of saliva for measuring progesterone as an indicator of luteal function during the menstrual cycle of women. A filtrate of saliva is collected in the mouth by means of an osmotic pump that accumulates medium containing only molecules less than 12,000 Da. We analyzed the nonextracted ultrafiltrate by a solid-phase immunoassay for progesterone and monitored the mid-luteal surge of lutropin in urine with a liquid-phase radioimmunoassay. Progesterone concentrations in the ultrafiltrate are significantly lower during the follicular phase and increase after the release of lutropin. The concentration of progesterone in the ultrafiltrate correlates closely with total progesterone in matched blood samples (r = 0.84, cycle 1; and r = 0.89, cycle 2). Likewise, we found a good correlation between the results in whole saliva and in the ultrafiltrate (r = 0.95). The described method of obtaining a pre- processed specimen noninvasively simplifies the self-collection of samples by patients (including collection at home); excludes potential interference from microorganisms, desquamated cells, and salivary components; and simplifies the processing of the biological fluid in the laboratory. en_US
dc.format.extent 35680 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher The American Association for Clinical Chemistry: Clinical Chemistry en_US
dc.subject Luteal function en_US
dc.subject Progesterone en_US
dc.subject Saliva testing en_US
dc.title Determination of free progesterone in an ultrafiltrate of saliva collected in situ en_US
dc.type Abstract en_US

Files in this item

Files Size Format View
ClinicalChemistryArchivePolicy.pdf 35.68Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search RIT DML


Advanced Search

Browse