Determination of free progesterone in an ultrafiltrate of saliva collected in situ

Show simple item record Schramm, W. en_US Smith, R. en_US Craig, Paul en_US Paek, S. en_US Kuo, H. en_US 2006-07-19T19:35:02Z en_US 2006-07-19T19:35:02Z en_US 1990-08-01 en_US
dc.identifier.citation Clinical Chemistry 36N8 (1990) 1488-1493 en_US
dc.identifier.issn 1530-8561 en_US
dc.identifier.uri en_US
dc.description Article may be found at: en_US
dc.description.abstract We have investigated the utility of an ultrafiltrate of saliva for measuring progesterone as an indicator of luteal function during the menstrual cycle of women. A filtrate of saliva is collected in the mouth by means of an osmotic pump that accumulates medium containing only molecules less than 12,000 Da. We analyzed the nonextracted ultrafiltrate by a solid-phase immunoassay for progesterone and monitored the mid-luteal surge of lutropin in urine with a liquid-phase radioimmunoassay. Progesterone concentrations in the ultrafiltrate are significantly lower during the follicular phase and increase after the release of lutropin. The concentration of progesterone in the ultrafiltrate correlates closely with total progesterone in matched blood samples (r = 0.84, cycle 1; and r = 0.89, cycle 2). Likewise, we found a good correlation between the results in whole saliva and in the ultrafiltrate (r = 0.95). The described method of obtaining a pre- processed specimen noninvasively simplifies the self-collection of samples by patients (including collection at home); excludes potential interference from microorganisms, desquamated cells, and salivary components; and simplifies the processing of the biological fluid in the laboratory. en_US
dc.format.extent 35680 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher The American Association for Clinical Chemistry: Clinical Chemistry en_US
dc.subject Luteal function en_US
dc.subject Progesterone en_US
dc.subject Saliva testing en_US
dc.title Determination of free progesterone in an ultrafiltrate of saliva collected in situ en_US
dc.type Abstract en_US

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