Identification and characterization of the nudix hydrolase from the Archaeon, Methanococcus jannaschii, as a highly specific ADP-ribose pyrophosphatase

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Title: Identification and characterization of the nudix hydrolase from the Archaeon, Methanococcus jannaschii, as a highly specific ADP-ribose pyrophosphatase
Author: Sheikh, Saifuddin; O'Handley, Suzanne; Dunn, Christopher; Bessman, Maurice
Abstract: The MJ1149 gene from the Archaeon, Methanococcus jannaschii, has been cloned and expressed in Escherichia coli. The 19-kDa protein containing the Nudix box, GX5EX7REUXEEXGU, has been purified and identified as a highly specific enzyme catalyzing the Mg2+-dependent hydrolysis of ADP-ribose according to the equation: ADP-ribose + H2O -> AMP + ribose-5-phosphate. The enzyme retains full activity when heated to 80 °C, and the rate of hydrolysis is 15-fold higher at 75 °C than at 37 °C in keeping with the thermophilicity of the organism. This is the first Nudix hydrolase identified from the Archaea, indicating that the family of enzymes containing the Nudix signature sequence is represented in all three kingdoms.
Record URI: http://hdl.handle.net/1850/2202
Publishers URL: http://dx.doi.org/10.1074/jbc.273.33.20924
Date: 1998-08-14

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