Papillomavirus DNA replication

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dc.contributor.author Ferran, Maureen en_US
dc.contributor.author McBride, Alison en_US
dc.date.accessioned 2006-08-14T15:39:35Z en_US
dc.date.available 2006-08-14T15:39:35Z en_US
dc.date.issued 1999-09 en_US
dc.identifier.citation Methods in Molecular Medicine 24 (1999) 341-360 en_US
dc.identifier.isbn 1-59259-245-7 en_US
dc.identifier.uri http://hdl.handle.net/1850/2283 en_US
dc.description.abstract Papillomavirus genomes replicate and are maintained as stable extrachromosomal plasmid DNA (episomes) in many cell lines. This process requires the viral E1 and E2 proteins and the origin of replication. The minimal origin of replication consists of an E1 binding site, an E2 binding site, and an AT rich region that probably facilitates origin unwinding. The E1 protein is an ATP-dependent helicase that specifically binds to and unwinds the origin. The E2 protein is the major transcriptional transactivator of the virus but it is also required for viral DNA replication. The E2 protein probably plays more of an auxiliary role in DNA replication; it has been shown to cooperatively bind to the origin with the E1 protein, to alleviate repression of replication by nucleosomes, and to interact with cellular replication proteins (RPA). To date, the most successful antiviral targets have been directed against viral-specific enzymes. Therefore, the ATPase and helicase activities of the E1 protein are attractive targets. Papillomavirus DNA replication may also be inhibited by compounds that interfere with the ability of E1 to bind DNA or to interact with the E2 protein. en_US
dc.format.extent 35612 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Humana Press: Methods in Molecular Medicine en_US
dc.relation.ispartofseries Methods in Molecular Medicine en_US
dc.relation.ispartofseries 24 en_US
dc.subject DNA replication en_US
dc.subject Papillomavirus en_US
dc.subject Viral proteins en_US
dc.title Papillomavirus DNA replication en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1385/1-59259-245-7:341

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