Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay

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Title: Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay
Author: Finch, Robert; Evans, Irene; Bosmann, H. Bruce
Abstract: The observation that cells often respond to carcinogens by nuclear enlargement has suggested that this property might be useful to develop a short-term screening test for such compounds. Previous methods for detecting nuclear size increases have used an image analyzer system to detect nuclear changes in individual cells. This paper details a more rapid method for obtaining nuclei by use of a stromalyzing procedure following by analysis of nuclear volumes, using a Coulter Counter Channelyzer. This new and simplified nuclear sizing method should facilitate the use of the assay as a possible carcinogenesis screen by permitting rapid and efficient testing of large numbers of compounds.
Record URI: http://hdl.handle.net/1850/2286
Publishers URL: http://dx.doi.org/10.1016/0300-483X(80)90008-6
Date: 1980

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