23Na and 39K nuclear magnetic resonance studies of perfused rat hearts. Discrimination of intra- and extracellular ions using a shift reagent

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dc.contributor.author Pike, Martin en_US
dc.contributor.author Frazer, J. en_US
dc.contributor.author Dedrick, D. en_US
dc.contributor.author Ingwall, J. en_US
dc.contributor.author Allen, P. en_US
dc.contributor.author Springer, Charles Jr. en_US
dc.contributor.author Smith, Thomas en_US
dc.date.accessioned 2006-08-18T20:54:27Z en_US
dc.date.available 2006-08-18T20:54:27Z en_US
dc.date.issued 1985-07 en_US
dc.identifier.citation Biophysical Journal 48N1 (1985) 159-173 en_US
dc.identifier.issn 1542-0086 en_US
dc.identifier.uri http://hdl.handle.net/1850/2344 en_US
dc.description.abstract High-resolution 23Na and 39K nuclear magnetic resonance (NMR) spectra of perfused, beating rat hearts have been obtained in the absence and presence of the downfield shift reagent Dy(TTHA)3- in the perfusing medium. Evidence indicates that Dy(TTHA)3- enters essentially all extracellular spaces but does not enter intracellular spaces. It can thus be used to discriminate the resonances of the ions in these spaces. Experiments supporting this conclusion include interventions that inhibit the Na+/K+ pump such as the inclusion of ouabain in and the exclusion of K+ from the perfusing medium. In each of these experiments, a peak corresponding to intracellular sodium increased in intensity. In the latter experiment, the increase was reversed when the concentration of K+ in the perfusing medium was returned to normal. When the concentration of Ca2+ in the perfusing medium was also returned to normal, the previously quiescent heart resumed beating. In the beating heart where the Na+/K+ pump was not inhibited, the intensity of the intracellular Na+ resonance was less than 20% of that expected. Although the data are more sparse, the NMR visibility of the intracellular K+ signal appears to be no more than 20%. en_US
dc.format.extent 26866 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Biophysical Society: Biophysical Journal en_US
dc.subject Heart en_US
dc.subject Nuclear magnetic resonance en_US
dc.subject Shift reagents en_US
dc.title 23Na and 39K nuclear magnetic resonance studies of perfused rat hearts. Discrimination of intra- and extracellular ions using a shift reagent en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1016/S0006-3495(85)83769-3

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