Separation, detection, and quantification of imine-linked opines by high-performance liquid chromatography

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Title: Separation, detection, and quantification of imine-linked opines by high-performance liquid chromatography
Author: Zhang, Dunhua; Shelby, Richard; Savka, Michael; Dessaux, Yves; Wilson, Mark
Abstract: Protocols for reversed-phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection were developed for the separation, detection and quantification of imine-linked opines, following 4-fluoro-7-nitrobenzoxadiazole (NBD-F) derivatization. Both natural opines (mannopine, mannopinic acid, cucumopine, octopine, octopinic acid, and nopaline) and opine analogs (nor-mannopine, galactopine, glucopine) were included in the study. The on-column detection limits for NBD-opines varied from 0.1 pmol to 5 pmol. These methods were applied to quantify mannopine on the leaf surfaces of transgenic tobacco (Nicotiana tabacum) plants expressing mannityl opine synthesis (mas) genes from Agrobacterium tumefaciens.
Record URI: http://hdl.handle.net/1850/2526
Publishers URL: http://dx.doi.org/10.1016/S0021-9673(98)00346-X
Date: 1998-07-17

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