Tobacco etch virus leader sequence enhances inducible indoleacetic acid-lysine synthetase activity in transgenic plants

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dc.contributor.author Savka, Michael en_US
dc.contributor.author Black, Robert en_US
dc.contributor.author Binns, Andrew en_US
dc.date.accessioned 2006-08-28T20:03:12Z en_US
dc.date.available 2006-08-28T20:03:12Z en_US
dc.date.issued 2001-07 en_US
dc.identifier.citation Plant Physiology and Biochemistry 39N7 (2001) 631-641 en_US
dc.identifier.issn 0981-9428 en_US
dc.identifier.uri http://hdl.handle.net/1850/2527 en_US
dc.description.abstract Indole-3-acetic acid (IAA) is one of several plant hormones that interact to coordinate growth and differentiation in higher plants. IAA has been identified in all species investigated and is found not only in its free form but also as sugar ester, peptide and amino acid conjugates. Plant processes ranging from the induction of cell division and expansion to the maintenance of apical dominance are dependent on free IAA levels. The objective of this study was to construct and characterize transgenic plants that contain the tetracycline (Tc)-specific inducible system to control the expression of the IAA-lysine synthetase gene (iaaL). The enzyme activity (IAA-L) encoded by iaaL conjugates free IAA with lysine to produce a foreign inactive conjugate IAA-lysine (IAA-Lys). Characterization of independent tobacco (Nicotiana tabacum cv. Wisconsin 38) transformants identified inducible plants completely repressed in the absence of tetracycline and derepressed to high IAA-L levels in the presence of inducer. The incorporation of the tobacco etch virus non-translated leader sequence (NTR) in context of the tetracycline-regulatable CaMV 35S triple-op promoter enhanced IAA-L activity 7-fold. Unlike tetracycline, the tetracycline analog anhydrotetracycline (ATc) did not inhibit the growth of callus at concentrations up to 50<space> g.mL-1 on solid medium. In the selected plants, IAA-Lys accumulation is dose-dependent upon the presence of the inducer molecule, and IAA-L activity in the derepressed state is similar to a plant that constitutively expresses iaaL at high levels from the native CaMV 35S promoter. en_US
dc.description.sponsorship This work was partially supported by Faculty Development Funds (FEAD) and Dean’s Summer Research Fellowship (DSRF) from the College of Science, Rochester Institute of Technology, Rochester, NY to MAS and by NIH grant GM47369-01 to ANB. en_US
dc.format.extent 37365 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Elsevier: Plant Physiology and Biochemistry en_US
dc.subject Genetic engineering en_US
dc.subject Phytohormones en_US
dc.subject Tobacco en_US
dc.title Tobacco etch virus leader sequence enhances inducible indoleacetic acid-lysine synthetase activity in transgenic plants en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1016/S0981-9428(01)01272-4

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