A liquid culture method for rescuing immature embryos of eastern cottonwood

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dc.contributor.author Savka, Michael en_US
dc.contributor.author Dawson, Jeffrey en_US
dc.contributor.author Jokela, Jalmer en_US
dc.contributor.author Skiryin, Robert en_US
dc.date.accessioned 2006-08-28T20:04:07Z en_US
dc.date.available 2006-08-28T20:04:07Z en_US
dc.date.issued 1987-01 en_US
dc.identifier.citation Plant Cell, Tissue and Organ Culture 10N3 (1987) 221-226 en_US
dc.identifier.issn 1573-5044 en_US
dc.identifier.uri http://hdl.handle.net/1850/2531 en_US
dc.description.abstract A one-step method to rescue immature embryos of eastern cottonwood (Populus deltoides Bartr.) is described. Plantlets developed from 83% of 25-day-old embryos grown in shaken culture on Murashige and Skoog (MS) liquid medium with 2.2 u-m indole-3-acetic acid (IAA) and from 86% of embryos not supplemented with IAA. In contrast, when the MS medium was solidified with 0.8% agar, plantlets developed from 25% of 25-day-old embryos cultured on medium supplemented with IAA and from 28% of embryos in medium not supplemented with IAA. Eighty eight percent of all plantlets survived a gradual acclimitization to peat plugs in a greenhouse. The one-step liquid-culture method is an effective means of rescuing immature embryos by ovule culture from excised artificially-pollinated female branches in our cottonwood breeding program. en_US
dc.format.extent 43151 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Springer: Plant Cell, Tissue and Organ Culture en_US
dc.subject Eastern cottonwood en_US
dc.subject Embryo culture en_US
dc.subject Liquid culture en_US
dc.title A liquid culture method for rescuing immature embryos of eastern cottonwood en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1007/BF00037306

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