An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays.

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dc.contributor.author Dietzgen, Ralf en_US
dc.contributor.author Snoke, Roy en_US
dc.contributor.author Babb, Bruce en_US
dc.date.accessioned 2006-08-29T18:37:08Z en_US
dc.date.available 2006-08-29T18:37:08Z en_US
dc.date.issued 1987-08-01 en_US
dc.identifier.citation Analytical Biochemistry 164N2 (1987) 297-302 en_US
dc.identifier.issn 0003-2697 en_US
dc.identifier.uri http://hdl.handle.net/1850/2576 en_US
dc.description.abstract A colorimetric reagent, 4-(4'-nitro-2'-methylsulfonylphenylazo)phenyl phosphate (NMPP), has been shown to be an effective substrate of alkaline phosphatase. NMPP and p-nitrophenyl phosphate were applied in comparative studies using enzyme immunoassays for the detection of viral antigens and antiviral antibodies. The new substrate exhibited similar, or even higher, sensitivity than p-nitrophenyl phosphate depending on the substrate concentrations used. Positive and negative reactions were easier to define, even without cumbersome equipment. The enzyme reaction was terminated by the uncompetitive inhibitor, theophylline. en_US
dc.format.extent 37365 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Elsevier: Analytical Biochemistry en_US
dc.subject Colorimetric reagents en_US
dc.subject Enzyme assays en_US
dc.subject Immunoassays en_US
dc.title An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays. en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1016/0003-2697(87)90496-9

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