A microfluidic cartridge to prepare spores for PCR analysis

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Title: A microfluidic cartridge to prepare spores for PCR analysis
Author: Belgrader, Phillip; Okuzumi, Margaret; Pourahmadi, Farzad; Borkholder, David; Northrup, M. Allen
Abstract: A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into PCR trube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.
Description: RIT community members may access full-text via RIT Libraries licensed databases: http://library.rit.edu/databases/
Record URI: http://hdl.handle.net/1850/5560
Publishers URL: http://dx.doi.org/10.1016/S0956-5663(99)00060-3
Date: 2000

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