Microchip isoelectric focusing using a miniature scanning detection system

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dc.contributor.author Raisi, Fariba en_US
dc.contributor.author Belgrader, Phillip en_US
dc.contributor.author Borkholder, David en_US
dc.contributor.author Herr, Amy en_US
dc.contributor.author Kintz, Gregory en_US
dc.contributor.author Pourahmadi, Farzad en_US
dc.contributor.author Taylor, Michael en_US
dc.contributor.author Northrup, M. Allen en_US
dc.date.accessioned 2008-02-15T16:39:32Z en_US
dc.date.available 2008-02-15T16:39:32Z en_US
dc.date.issued 2001 en_US
dc.identifier.citation Electrophoresis 22 (2001) 2291-2295 en_US
dc.identifier.uri http://hdl.handle.net/1850/5588 en_US
dc.description RIT community members may access full-text via RIT Libraries licensed databases: http://library.rit.edu/databases/
dc.description.abstract A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real-time distribution of fluorescently labeled peptides subjected to gel-free mIEF. During the run, the effective length of the 6-cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements. en_US
dc.description.sponsorship This project was funded by the Advanced Diagnostics for Pathogens Program of the Defense Advanced Research project Agency (DARPA) under contract No. N653236-98-1-5414. en_US
dc.language.iso en_US en_US
dc.publisher John Wiley & Sons Ltd. en_US
dc.subject Microchip isoelectric focusing en_US
dc.subject Miniature scanning detector en_US
dc.title Microchip isoelectric focusing using a miniature scanning detection system en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1002/1522-2683(20017)22:11<2291::AID-ELPS2291>3.0.CO;2-A

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